DNA Extraction and Purification
New England Biolabs, Inc. Monarch® Spin PCR & DNA Cleanup Kit - 50 preps
The Monarch Spin PCR & DNA Cleanup Kit (5 ug), a newer version of our original PCR cleanup kit, offers an efficient method for purification and concentration of up to 5 ug of high-quality, double-stranded and single-stranded DNA from enzymatic reactions such as PCR, restriction digestion, ligation, and reverse transcription. This new version features upgraded spin columns precision-engineered to uniquely allow for low elution, in as little as 5 ul, for highly concentrated DNA in downstream applications such as sequencing, restriction digestion, library construction, labeling and other enzymatic manipulations. Our optimized buffer system allows efficient binding and purification of the DNA, without a need to monitor pH. These kits contain uniquely designed columns and bottles to significantly reduce plastic usage compared to leading suppliers, while maintaining structural integrity for centrifugation, vacuum manifolds and other steps
Zymo Research Corporation Quick-DNA Plant/Seed 96 Kit (2 x 96 Preps)
The procedure is easy and can be completed in as little as 50 minutes: plant samples ( 80 mg each) are added directly to the tubes of a ZR BashingBead™ Lysis Rack (2.0 mm) and are rapidly and efficiently lysed by bead beating (e.g., 2010 GenoGrinder Instrument, page 5) without the use of organic denaturants or proteinases. Polysaccharides and polyphenols/tannins are removed from the DNA using our Zymo-Spin™ 96-well plate and Silicon-A™-HRC Plate technologies, respectively. The eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, etc.
Research Products International Corp ZR Plasmid Miniprep-Classic Kit (800 Preps)
The ZR Plasmid Miniprep-Classic is designed for efficient isolation of plasmid DNA from E. coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, and reliable. It features a modified alkaline lysis protocol together with Zymo-Spin technology to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy visualization of complete cell lysis and neutralization. The innovative Zymo-Spin llN columns yield transfection-grade plasmid DNA. Plasmid DNA purified using the ZR Plasmid Miniprep-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.
High Purity: Eluted plasmid DNA is high quality, endotoxin-free and ready for all sensitive downstream applications including transfections and sequencing.
Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization.
Plasmid MegaPrep kit, 16 samples, yield up to 5 mg, endotoxin level <0.1 EU/μg, supercoiled DNA, elution volume 18 mL, A260/A280 purity >1.8, A260/A230 purity >2.0, compatible with Biotage PhyPrep system
Plasmid MegaPrep kit, 16 samples, yield up to 5 mg, endotoxin level <0.1 EU/μg, supercoiled DNA, elution volume 18 mL, A260/A280 purity >1.8, A260/A230 purity >2.0, compatible with Biotage PhyPrep system
Macherey-Nagel MACHEREY-NAGEL NucleoBond HMW DNA Kit, High Integrity DNA Purification, Enzymatic Lysis Included, 20 Preps
MACHEREY-NAGEL NucleoBond HMW DNA kit is designed for the purification of high molecular weight DNA, ensuring high integrity suitable for long-read and Next Generation Sequencing applications. The kit utilizes anion-exchange chromatography and includes Proteinase K for gentle enzymatic lysis, minimizing DNA shearing. It is ideal for isolating DNA from cultured cells, tissue, and plant samples, making it a versatile choice for demanding research environments.
Zymo Research Corporation ZP Express™ Neutralization Buffer, 260 ml
ZP Express™ Neutralization Buffer, 260 ml
ATCC Quantitative Genomic DNA; from Mycobacterium tuberculosis; Strain H37Rv
ATCC Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load.
Zymo Research Corporation ZymoPURE P1 (Red), 150 ml
ZymoPURE™ P1 (Red) (150 ml). For use in ZymoPURE™ Plasmid Purification kits. Colored buffers permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
Boca Scientific Inc AquaGenomic DNA Extraction Solution, 30ml, 300 minipreps
AquaGenomic is a multifunctional aqueous reagent designed for simple, scalable, high yield genomic DNA extraction. AquaGenomic can be used with all types of specimen, from bacteria to plant and animal tissues. 9 different extraction protocols are provided for different types of samples including cells, tissues, saliva, microbes, stool and soil, swabs, whole blood, white blood cells, and avian blood. Unlike column-based kits, DNA extraction with AquaGenomic requires only one solution, and one solution can be applied to multiple types of specimen. AquaGenomic is non-toxic and its lysate can be used for PCR directly without DNA purification. One 30ml bottle is sufficient for 300 minipreps.
Zymo Research Corporation ZymoBIOMICS™ Microbial Community DNA Standard (200 ng)
One of the major challenges in the emerging field of microbiomics is the bias and errors introduced in the complex workflows. Besides nucleic acid purification, bias also arises from sequencing library preparation and subsequent processes. The ZymoBIOMICS Microbial Community DNA Standard is designed to assess bias, errors and other artifacts after the step of nucleic acid purification. This DNA standard is created by pooling DNA extracted from pure cultures. It has an accurately defined composition, negligible impurities (0.01%), and contains genomes of a wide range of GC content (15% - 85%). This DNA standard is designed to have the same microbial composition with the cellular version, the ZymoBIOMICS Microbial Community Standard, so that they can be more powerful when working in tandem.
Sigma Aldrich Fine Chemicals Biosciences Inactivated Escherichia coli Suitable for DNA extraction, PCR, sequencing, next generation sequencing, >10^8 bacteria/ml | 1ML
Inactivated Escherichia coli Suitable for DNA extraction, PCR, sequencing, next generation sequencing, >10^8 bacteria/ml | 1ML